Co-elevation of atmospheric CO2 and temperature affect instantaneous and intrinsic water use efficiency of rice varieties


  • PARTHA PRATIM MAITY Division of Environmental Sciences, ICAR-IARI, New Delhi-110 012, India
  • BIDISHA CHAKRABARTI Division of Environmental Sciences, ICAR-IARI, New Delhi-110 012, India
  • A BHATIA Division of Environmental Sciences, ICAR-IARI, New Delhi-110 012, India
  • S N KUMAR Division of Environmental Sciences, ICAR-IARI, New Delhi-110 012, India
  • TJ PURAKAYASTHA Division of Soil Science and Agricultural Chemistry, ICAR-IARI, New Delhi-110 012, India
  • D CHAKRABORTY Division of Agricultural Physics, ICAR-IARI, New Delhi-110 012, India
  • S ADAK Division of Agricultural Physics, ICAR-IARI, New Delhi-110 012, India
  • A SHARMA Division of Environmental Sciences, ICAR-IARI, New Delhi-110 012, India
  • S KANNOJIYA Division of Environmental Sciences, ICAR-IARI, New Delhi-110 012, India



Elevated CO2, High temperature, Instantaneous WUE, Intrinsic WUE, Photosynthesis rate, Rice


Greenhouse gas (GHG) emissions from anthropogenic activities are the most significant drivers of climate change, which has both direct and indirect effects on crop production. The study was conducted during the kharif season for two years inside the Open Top Chamber (OTC) at the Genetic-H field of ICAR-Indian Agriculture Research Institute (IARI) to quantify the effect of elevated CO2 and temperature on water use efficiency of rice varieties. There were two different CO2 concentrations i.e. ambient (410 ppm) and elevated (550 ± 25 ppm) and also two different temperature levels i.e. ambient and elevated (+2.5-2.9°C). Results suggested that warming caused more accumulated GDD in rice and which negatively affected the duration of both the varieties. In elevated CO2 plus high temperature interaction treatment net photosynthesis rate was more than that of chamber control. Stomatal conductance and transpiration rate reduced with co-elevation of CO2 and temperature. Co-elevation of CO2 and temperature, has also improved WUE (both instantaneous and intrinsic) through enhanced carbon assimilation and reduced stomatal conductance, thus, reducing the amount of water lost through transpiration, eventually improving WUE of the crop.


Abdelhakim, L.O.A., Palma, C.F.F., Zhou, R., Wollenweber, B., Ottosen, C.O. and Rosenqvist, E. (2021). The effect of individual and combined drought and heat stress under elevated CO2 on physiological responses in spring wheat genotypes. Plant Physiol. Biochem., 162:301-314.

Ainsworth, E.A. and Rogers, A. (2007). The response of photosynthesis and stomatal conductance to rising [CO2]: mechanisms and environmental interactions. Plant Cell Environ., 30: 258–270.

Apple, M. E., Olszyk, D. M., Ormrod, D. P., Lewis, J., Southworth, D. and Tingey, D. T. (2000). Morphology and stomatal function of Douglas fir needles exposed to climate change: Elevated CO2 and temperature. Int. J. Plant Sci., 161: 127–132.

Brito, F. A., Pimenta, T. M., Henschel, J. M., Martins, S. C., Zsögön, A. and Ribeiro, D. M. (2020). Elevated CO2 improves assimilation rate and growth of tomato plants under progressively higher soil salinity by decreasing abscisic acid and ethylene levels. Environ. Exp. Bot., 176: 104050.

Bunce, J. A. (2004). Carbon dioxide effects on stomatal responses to the environment and water use by crops under field conditions. Oecologia, 140: 1–10.

Cai, C., Yin, X., He, S., Jiang, W., Si, C., Struik, P.C., Luo, W., Li, G., Xie, Y., Xiong, Y. and Pan, G. (2016). Responses of wheat and rice to factorial combinations of ambient and elevated CO2 and temperature in FACE experiments. Glob. Change Biol., 22: 856–874.

Chakrabarti B, Singh SD, Bhatia A, Kumar V and Harit RC (2020) Yield and Nitrogen Uptake in Wheat and Chickpea Grown Under Elevated Carbon Dioxide Level. Natl. Acad. Sci. Lett. 43, 109-113.

Chakrabarti, B., Bhatia, A., Pramanik, P., Singh, S.D., Jatav, R.S., Saha, N.D. and Kumar, V. (2021). Changes in thermal requirements, growth and yield of wheat under the elevated temperature. Indian J. Agric. Sci., 91(3):435-439.

Condon A.G., Richards R.A., Rebetzke G.J. and Farquhar G.D. (2004). Breeding for high water-use efficiency. J. Exp. Bot., 55: 2447-2460.

Das, P., Deka, R. L., Goswami, J. and Barua, S. (2020). Effect of elevated CO2 and temperature on growth and yield of winter rice under Jorhat condition. J. Agrometeorol., 22(2):109-115. DOI:

Dey, Sumit Kr., Chakrabarti B, Prasanna R, Mittal R, Singh SD and Pathak H. (2016). Growth and biomass partitioning in mungbean with elevated carbon dioxide, phosphorus levels and cyanobacteria inoculation. J. Agrometeorol.,18(1): 7-12. DOI:

Dwivedi, S.K., Kumar, S., Mishra, J.S., Prakash, V., Rao, K.K., Bhatt, B.P. and Srivastava, A.K. (2022). Interactive effect of elevated [CO2] and temperature on the photosynthetic process, anti‐oxidative properties, and grain yield of rice. J. Agron. Crop Sci., 208(3):384-393.

FAOSTAT (2021). Food and Agriculture Data. Food and Agriculture Organization of the United Nations, Rome.

Farquhar, G. and Richards, R. (1984). Isotopic composition of plant carbon correlates with water-use efficiency of wheat genotypes. Aust. J. Plant Physiol., 11(6): 539.

Guo, L., Yu, Z., Li, Y., Xie, Z., Wang, G., Liu, X., Liu, J., Liu, J. and Jin, J. (2022). Plant phosphorus acquisition links to phosphorus transformation in the rhizospheres of soybean and rice grown under CO2 and temperature co-elevation. Sci. Total Environ., 823: 153558.

Hatfield, J.L. and Dold, C. (2019). Water-use efficiency: advances and challenges in a changing climate. Front. Plant Sci., 10:103.

IPCC (2021). Summary for Policymakers, In: Climate Change, The Physical Science Basis. Contribution of Working Group I to the Sixth Assessment Report of the Intergovernmental Panel on Climate Change [Masson-Delmotte V P et al (eds)]. Cambridge University Press, Cambridge, United Kingdom and New York, NY, USA, p 3−32.

Kumar R, Ramesh K, Singh R D and Prasad R. (2010).Modulation of wild marigold (Tagetesminuta L.) phenophases towards the varying temperature regimes - A field study. J. Agrometeorol.,12(2): 234–40. DOI:

Kumar, A., Nayak, A.K., Das, B.S., Panigrahi, N., Dasgupta, P., Mohanty, S., Kumar, U., Panneerselvam, P. and Pathak, H. (2019). Effects of water deficit stress on agronomic and physiological responses of rice and greenhouse gas emission from rice soil under elevated atmospheric CO2. Sci. Total Environ., 650:2032-2050.

Lahr, E. C., Schade, G. W., Crossett, C. C. and Watson, M. R. (2015). Photosynthesis and isoprene emission from trees along an urban–rural gradient in Texas. Global Change Biol., 21: 4221–4236.

Lenka, N. K., Lenka, S., Yashona, D. S., Shukla, A. K., Elanchezhian, R. and Dey, P. (2021). Carbon dioxide and/or temperature elevation effect on yield response, nutrient partitioning and use efficiency of applied nitrogen in wheat crop in central India. Field Crop Res., 264: 108084.

Li, Yuping, Li, H., Li, Yuanyuan and Zhang, S. (2017). Improving water-use efficiency by decreasing stomatal conductance and transpiration rate to maintain higher ear photosynthetic rate in drought-resistant wheat. Crop J., 5: 231–239.

Li, X., Kang, S., Niu, J., Huo, Z. and Liu, J. (2019). Improving the representation of stomatal responses to CO2 within the penman–monteith model to better estimate evapotranspiration responses to climate change. J. Hydrol., 572: 692–705.

Nuttonson M Y. (1955). Wheat climatic relationship and use of phenology in ascertaining the thermal and photothermal requirements of wheat. Am. Inst. Crop Ecol., Washington D.C.

Oiao, Y., Zhang, H., Dong, B., Shi, C., Li, Y., Zhai, H. and Liu, M. (2010). Effect of elevated CO2 concentration on growth and water use efficiency of winter wheat under two soil water regimes. Agric Water Manag., 97:1742–1748.

Osmond C.B., Bjorkman O. and Anderson D.J. (1980). Physiological Processes in Plant Ecology. Toward a SynthesisWith Atriplex. Springer Verlag, Berlin, Germany.

Pathak, H., Samal, P. and Shahid, M. (2018). Revitalizing rice-systems for enhancing productivity, profitability and climate resilience. In: Pathak, H., et al. (Eds.), Rice Research for Enhancing Productivity, Profitability and Climate Resilience. ICAR-National Rice Research Institute, Cuttack, Odisha, pp. 1–17.

Pazzaglia, P.T., Weiner, J. and Liu, F. (2016). Effects of CO2 elevation and irrigation regimes on leaf gas exchange plant water relations, and water use efficiency of two tomato cultivars. Agric. Water Manag., 169: 26–33.

Qiao, Y., Zhang, H., Dong, B., Shi, C., Li, Y., Zhai, H. and Liu, M. (2010). Effects of elevated CO2 concentration on growth and water use efficiency of winter wheat under two soil water regimes. Agric. Water Manag., 97: 1742–1748.

Raj A, Chakrabarti B, Pathak H, Singh SD, Mina U and Mittal R (2016). Growth, yield components and grain yield response of rice to temperature and nitrogen levels. J. Agrometeorol.,18(1): 1-6. DOI:

Ruiz-Vera, U.M., Siebers, M., Gray, S.B., Drag, D.W., Rosenthal, D.M., Kimball, B.A., Ort, D.R. and Bernacchi, C.J. (2013). Global warming can negate the expected CO2 stimulation in photosynthesis and productivity for soybean grown in the Midwestern united states. Plant Physiol., 162: 410–423.

Sandhu, S.S., Kaur, P., Gill, K.K. and Bal, S.K. (2017). Effect of high temperature on performance ofrice and its management. J. Agric. Phys.,17(2): 249-254.

Saxena, R. and Kumar, S. N. (2014). Simulating the impact of projected climate change on rice (Oryza sativa L.) yield and adaptation strategies in major rice growing regions of India. J. Agrometeorol., 16(1): 18-25. DOI:

Singh, P. (2023). Crop models for assessing impact and adaptation options under climate change. J. Agrometeorol., 25(1): 18-33. DOI:

Urban, J., Ingwers, M. W., McGuire, M. A. and Teskey, R. O. (2017). Increase in leaf temperature opens stomata and decouples net photosynthesis from stomatal conductance in Pinustaeda and Populusdeltoides x nigra. J. Exp. Bot., 68: 1757- 1767.

Wang, J., Liu, X., Zhang, X., Smith, P., Li, L., Filley, T.R., Cheng, K., Shen, M., He, Y. and Pan, G. (2016). Size and variability of crop productivity both impacted by CO2 enrichment and warming–a case study of 4 year field experiment in a Chinese paddy. AgrEcosyst. Environ., 221: 40–49.

Zhang, C., Li, Y., Yu, Z., Wang, G., Liu, X., Liu, J., Liu, J., Zhang, X., Yin, K. and Jin, J. (2022). Co-elevation of atmospheric [CO2] and temperature alters photosynthetic capacity and instantaneous water use efficiency in rice cultivars in a cold-temperate region. Front. Plant Sci., 13.

Zhu, C., Langley, J. A., Ziska, L. H., and Cahoon, D. R. (2022). Accelerated sealevel rise is suppressing CO2 stimulation of tidal marsh productivity: A 33-year study. Sci. Adv. 8: eabn0054.




How to Cite

PARTHA PRATIM MAITY, CHAKRABARTI, B., BHATIA, A., S N KUMAR, PURAKAYASTHA, T., CHAKRABORTY, D., ADAK, S., SHARMA, A., & KANNOJIYA, S. (2023). Co-elevation of atmospheric CO2 and temperature affect instantaneous and intrinsic water use efficiency of rice varieties. Journal of Agrometeorology, 25(3), 404–409.

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